High Performance Liquid Chromatography (HPLC)
The Comprehensive Course to Become Professional in High Performance Liquid Chromatography (HPLC) High Performance Liquid Chromatography (HPLC)
Courses to get you started
What you'll learn
- Understand and define basics of chromatography by learning stationary phase, mobile phase and analyte
- Explain chromatography principles by understanding of affinity theory (solubility & adsorption)
- Explain the types of chromatography based on bed type, separation mechanism, and physical states of phases
- Understand instrumental design of HPLC system by explaining HPLC compartments
- Define mission and importance of HPLC compartments such as solvent reservoir, solvent delivery system (pump), sampler unit & injector, column, dedector, monitor
- Evaluate mission of HPLC techniques in analytical chemistry by explaining qualitative and quantitative analysis
- Define types of HPLC such as Normal Phase (NP HPLC), Reversed Phase (RP HPLC), Ion Exchange (IEX) and Size Exclusion Chromatography (SEC)
- Evaluate and understand descriptors of HPLC such as Retention Time, Void Volume, Retention Factor, Selectivity, Efficiency (Teorical Plate Number), Resolution
Requirements
- Require mobile/tablet/laptop/personal computer with internet
- To be student, graduate and/or chemistry/pharma professional
High Performance Liquid Chromatography (HPLC) techniques are so popular and common in pharma industry, cosmetic industry, chemistry industry, food industry, polymer industry and environment laboratories.
High performance liquid chromatography (HPLC) is the crucial and integral analytical tool applied in all stages of drug discovery, development, and commercialization in the pharmaceutical industry, especially. At the each step of drug development, drug substances, ingredients and drug products are controlled and monitored by using HPLC techniques.
In this course, chromotography, chromatography principles, types of chromatography, instrumental design of HPLC, HPLC Analysis, Types of HPLC and Descriptors of HPLC have been discussed within all technical details.
HIGH PERFORMANCE LIQUID CHROMATOGRAPHY (HPLC) COURSE SCOPE
1 INTRODUCTION
2 INTRODUCTION TO HIGH PERFORMANCE LIQUID CHROMATOGRAPHY (HPLC)
3 CHROMATOGRAPHY
3_1 History of Chromatography
3_2 What is Chromatography?
3_3 What is Stationary Phase?
3_4 What is Mobile Phase?
3_5 What is Analyte?
4 CHROMATOGRAPHY PRINCIPLES
4_1 Chromatographic Seperation
4_2 Chromatography (Affinity & Interaction)
4_3 Chromatography (Solubility & Adsorption)
4_4 Chromatography (Solubility & Polarity)
4_5 Chromatography (Solubility & Hidrophilic / Hidrophobic)
4_6 Let's Categorize Molecules!
5 TYPES OF CHROMATOGRAPHY
5_1 Chromatography Classification Based on Bed (Column Chromatography, Paper Chromatogrphy, Thin Layer Chromatography)
5_2 Chromatography Classification Based on Separation Mechanism (Affinity Chromatography, Ion Exchange Chromatography, Gel Filtration Chromatography)
5_3 Chromatography Classification Based on Physical States of Phases (Gas Chromatography, Liquid Chromatography)
6 INSTRUMENTAL DESIGN OF HPLC SYSTEM
6_1 Instrumental Design of HPLC System_Simple Technical Design
6_2 Instrumental Design of HPLC System_Technical Design
6_3 Instrumental Design of HPLC System_Equipment Compartments
6_4 Instrumental Design of HPLC System_Solvent Reservoir
6_5 Instrumental Design of HPLC System_Solvent Delivery System (Pump)
6_6 Instrumental Design of HPLC System_Sampler Unit & Injector & Autosampler
6_7 Instrumental Design of HPLC System_Column
6_8 Instrumental Design of HPLC System_Dedector
6_9 Instrumental Design of HPLC System_PC Monitor
7 HPLC ANALYSIS
7_1 HPLC in Analytical Chemistry
7_2 Qualitative Analysis
7_3 Quantitative Analysis
7_4 Quantitative Analysis Example
8 TYPES OF HPLC
8_1 Types of HPLC
8_2 Normal Phase HPLC (NP HPLC)
8_3 Reversed Phase HPLC (RP HPLC)
8_4 Ion Exchange Chromatography (IEX)
8_5 Size Exclusion Chromatography (SEC)
9 DESCRIPTORS OF HPLC & ANALYTICAL METHOD DEVELOPMENT
9_1 Retention Time
9_2 Void Volume (Dead Volume)
9_3 Retention Factor
9_4 Selectivity
9_5 Efficiency (Teorical Plate Number)
9_6 Selectivity & Efficiency
9_7 Resolution
10 CONCLUSION
Who this course is for:
- Anyone who is willing to learn High Performance Liquid Chromatography (HPLC)
- Anyone who wants to learn the chemistry of the High Performance Liquid Chromatography (HPLC)
- Anyone who wants to be qualified in analytical chemistry, instrumental analysis and pharmaceutical industry
- Anyone who need to use HPLC techniques in analytical chemistry and/or pharmacy in the learning process
- Anyone who desire to be master in HPLC analysis by using technical/chemical background
- Anyone who wants to get certification related with High Performance Liquid Chromatography (HPLC)
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